Stainless steel electrode for sensitive luminol electrochemiluminesce

Zholudov, Yu.; Shimeles Addisu Kitte; Wenyue Gao; Anaclet Nsabimana; Zhongyuan Liu; Guobao Xu; Liming Qi

Публікація:
Stainless steel electrode for sensitive luminol electrochemiluminesce

dc.contributor.author	Zholudov, Yu.
dc.contributor.author	Shimeles Addisu Kitte
dc.contributor.author	Wenyue Gao
dc.contributor.author	Anaclet Nsabimana
dc.contributor.author	Zhongyuan Liu
dc.contributor.author	Guobao Xu
dc.contributor.author	Liming Qi
dc.date.accessioned	2017-11-03T07:13:27Z
dc.date.available	2017-11-03T07:13:27Z
dc.date.issued	2017
dc.description.abstract	The electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. The type 304 stainless steel electrode shows appealing ECL performance for luminol-H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of0.456 nM (S/N = 3). The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective,and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, the applications of stainless steel electrode-based ECL for the detection of enzyme activities and enzyme substrates were further investigated using glucoseoxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to the ECL intensities over a range of 0.1 – 1000 µM and 0.001 – 0.7 U/mL with a limit of detections of 0.076 μM and 0.00087 U/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of its remarkable performance and user-friendly features, stainless steel electrode holds great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.	uk_UA
dc.identifier.citation	Stainless steel electrode for sensitive luminol electrochemiluminescence detection of H2O2, glucose, and glucose oxidase activity Shimeles Addisu Kitte, Wenyue Gao, Yuriy T. Zholudov, Liming Qi, Anaclet Nsabimana, Zhongyuan Liu, and Guobao Xu Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.7b01939 • Publication Date (Web): 23 Aug 2017	uk_UA
dc.identifier.uri	http://openarchive.nure.ua/handle/document/4067
dc.language.iso	en	uk_UA
dc.publisher	Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES	uk_UA
dc.subject	chemiluminescence electrogenerated	uk_UA
dc.subject	H2O2 detection
dc.subject	stainless steel electrode
dc.title	Stainless steel electrode for sensitive luminol electrochemiluminesce	uk_UA
dc.type	Article	uk_UA
dspace.entity.type	Publication