Перегляд за автором "Zholudov, Yu."
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Публікація Electrochemiluminescence in Tryptophan / Tetraphenylborate System for Biosamples Assay(ISBC, 2018) Zholudov, Yu.; Lysak, N.; Xu, G.L-tryptophan is one of the most important essential amino acids which role in the human body is invaluable. It participates in maintaining of nitrogen balance in metabolism, in the synthesis of muscle proteins, enzymes, antibodies of the immune system and other biologically active substances. Thus the development of reliable methods for quantitative determination of this compound in biolsamples, foods and medicines is an actual task for today.Публікація Electrogenerated chemiluminescence in tryptophan / tetraphenylborate system for biosamples assay(КрНУ, 2018) Zholudov, Yu.In this work the results of study of electrochemiluminescence in the system tryptophan / co-reatant tetraphenylborate and analysis of the possibility of its use for measuring the content of the amino acid tryptophan in biological samples are presented.Публікація Langmuir–Blodgett technique versatility for electrode modification and its ECL application(ISBC 2018, 2018) Snizhko, D.; Zholudov, Yu.; Kukoba, A.; Xu, G.In the work 9,10-diphenyl anthracene and rubrene were used to decorate an amphiphilic polymer matrix polymethylmethacrylate (PMMA) lm. ITO electrodes equipped with LB lm were used in ECL experiment with tripropylamine co-reactant. Obtained ECL response satis es expectation in looking for a new technology for ECL sensor fabrication.Публікація Physics [Text], Part 1. Mechanics, Thermodynamics, Vibration and Waves, Electricity and Nagnetism: Summary of Letures.(SMIT, 2005) Rozhitskii, M. M.; Churilov, O.; Горбачев, В. А.; Zholudov, Yu.Публікація Physics [Text], Part 2. Electric Circuits, Optics and Quantum Mechanics: Summary of Letures(SMIT, 2006) Rozhitskii, M. M.; Churilov, O.; Zholudov, Yu.; Горбачев, В. А.Публікація Stainless steel electrode for sensitive luminol electrochemiluminesce(Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES, 2017) Zholudov, Yu.; Shimeles Addisu Kitte; Wenyue Gao; Anaclet Nsabimana; Zhongyuan Liu; Guobao Xu; Liming QiThe electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. The type 304 stainless steel electrode shows appealing ECL performance for luminol-H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of0.456 nM (S/N = 3). The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective,and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, the applications of stainless steel electrode-based ECL for the detection of enzyme activities and enzyme substrates were further investigated using glucoseoxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to the ECL intensities over a range of 0.1 – 1000 µM and 0.001 – 0.7 U/mL with a limit of detections of 0.076 μM and 0.00087 U/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of its remarkable performance and user-friendly features, stainless steel electrode holds great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.Публікація System for two photon imaging of biosamples(Харків, ХНУРЕ, 2019) Aljebur Ali Salman; Zholudov, Yu.The work is devoted to development of scanning imaging system for bioobjects based on two-photon fluorescence of nanoparticles. It is based on the use of nanomaterials in imagining systems and some of nonfluorescent imaging applications and labeling with fluorescent nanoparticles. The basics of two photon fluorescence, its imaging application, properties of nanoparticles and biological tissues, used for our system development were analyzed. Based on this analysis we developed the general scheme of the system for scanning imaging of bioobjects using CdTe quantum dot labels and method of two-photon fluorescence.