Перегляд за автором "Shimeles Addisu Kitte"

Зараз показано 1 - 2 з 2
  • Зображення мініатюри
    Публікація
    Artemisinin-luminol chemiluminescence for forensic bloodstain detection using smart phone as detector
    (Subscriber access provided by TRENT UNIV, 2017) Muzyka, K.; Chao Wang; Wenyue Gao; Shimeles Addisu Kitte; Jianping Li; Wei Zhang
    Forensic luminol chemiluminescence test is one of the most sensitive and popular methods for the determination oflatent bloodstains. It mainly uses hydrogen peroxide or sodium perborate as coreactants. The easy decomposition of hydrogen peroxide and sodium perborate in the presence of many ions significantly affects the selectivity. Artemisinin is a natural peroxide that is quite stable in the presence of common ions. In the present study, artemisinin has been exploited for the forensic bloodstain chemiluminescence detection for the first time. Using smart phone as cost-effective portable detector, the visual detection of bloodstains has been achieved with a dilution factor of blood upto 100000. Moreover, this system shows excellent selectivity against many common species. It can well differentiate bloodstains from other stains, such as coffee, brown sugar, and black tea. Both favourable sensitivity and selectivity makes the present method promising in forensic detection.
  • Зображення мініатюри
    Публікація
    Stainless steel electrode for sensitive luminol electrochemiluminesce
    (Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES, 2017) Zholudov, Yu.; Shimeles Addisu Kitte; Wenyue Gao; Anaclet Nsabimana; Zhongyuan Liu; Guobao Xu; Liming Qi
    The electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. The type 304 stainless steel electrode shows appealing ECL performance for luminol-H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of0.456 nM (S/N = 3). The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective,and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, the applications of stainless steel electrode-based ECL for the detection of enzyme activities and enzyme substrates were further investigated using glucoseoxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to the ECL intensities over a range of 0.1 – 1000 µM and 0.001 – 0.7 U/mL with a limit of detections of 0.076 μM and 0.00087 U/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of its remarkable performance and user-friendly features, stainless steel electrode holds great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.