Перегляд за автором "Guobao Xu"

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  • Зображення мініатюри
    Публікація
    Progress and chellenges in electrochemiluminescent aptasensors
    (PII: S0956-5663(17)30015-5 DOI: http://dx.doi.org/10.1016/j.bios.2017.01.015 Reference: BIOS9480, 2017) Muzyka, K.; Muhammad Saqib; Zhongyuan Liu; Wei Zhang; Guobao Xu
    The importance of developing new diagnostic and detection technologies for the growing number of sensing challenges is rising each year. Here, we present a comprehensive and concise review on electrochemiluminescent (ECL) aptasensors by putting special emphasis on its characteristic features, advances, challenges, and applications of ECL based aptasensors. ECL is an ideal tool for constructing such sensors because of its inherent characteristics and can be easily integrated into aptamer based sensing platforms. This review summarizes the "synergistic benefits" of ECL aptamer-based sensors; classifications of ECL aptamer-based assay designs, and signal amplification strategies. This critical review highlights the effects of integration of nanomaterials, immobilization techniques, and amplification/detection strategies on the analytical performance of ECL based aptasensors. Moreover, several proof-of-concepts with appropriate figures and explanations have been shown to provide a general guide for the design of ECL aptasensors, and to stimulate further application of these ECL aptasensors. Finally, we conclude with the remaining challenges and opportunities to inspire further developments in ECL aptasensors.
  • Зображення мініатюри
    Публікація
    Stainless steel electrode for sensitive luminol electrochemiluminesce
    (Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES, 2017) Zholudov, Yu.; Shimeles Addisu Kitte; Wenyue Gao; Anaclet Nsabimana; Zhongyuan Liu; Guobao Xu; Liming Qi
    The electrogenerated chemiluminescence (ECL) application of stainless steel, a robust and cost-effective material, has been developed for the first time. The type 304 stainless steel electrode shows appealing ECL performance for luminol-H2O2 system. It enables the detection of H2O2 with a linear range from 1 to 1000 nM and a limit of detection of0.456 nM (S/N = 3). The ECL method based on type 304 stainless steel electrode is more sensitive, more cost-effective,and much simpler than other ECL methods reported before. Because the stainless steel electrode has excellent performance for H2O2 detection and H2O2 participates in many important enzymatic reactions, the applications of stainless steel electrode-based ECL for the detection of enzyme activities and enzyme substrates were further investigated using glucoseoxidase (GODx) and glucose as representative enzyme and substrate. The concentrations of glucose and the activity of GODx were directly proportional to the ECL intensities over a range of 0.1 – 1000 µM and 0.001 – 0.7 U/mL with a limit of detections of 0.076 μM and 0.00087 U/mL (S/N = 3), respectively. This method was successfully used for determining glucose in honey. Because of its remarkable performance and user-friendly features, stainless steel electrode holds great promise in various electroanalytical applications, such as biosensing, disposable sensors, and wearable sensors.
  • Зображення мініатюри
    Публікація
    Tris(2,2′-bipyridine)ruthenium(II) electrochemiluminescence using rongalite as coreactant and its application in detection of foodstuff adulteration,”
    (Journal of Electroanalytical Chemistry, 2020) Zholudov, Yu. T.; Abubakar Abdussalam; Fan Yuan; Fangxin Du; Muhammad Nadeem Zafar; Guobao Xu; Xiangui Ma
    The persistent reports on the indiscriminate contamination of foodstuffs with inedible chemicals for economic gains and the high cost of the existing analytical methods for their detection make it necessary to develop simple and cheaper methods for the immediate detection of those toxic substances. Here, we report a fast and highly sensitive electrochemiluminescence (ECL) method for the detection of foodstuffs adulteration with an industrial bleaching agent-rongalite (RGL) for the first time. Under the optimal conditions, the ECL intensity of the tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)3 2+) increases linearly with the concentration of RGL in the range of 1 μM to 1000 μM with a limit of detection of 0.57 μM (S/N = 3). The developed method has shown excellent selectivity in the presence of co-existing interfering species. The application of the ECL method in this contribution can be extended to other foodstuffs that might be adulterated with RGL other than the one (tofu) investigated here.