System for two photon imaging of biosamples

Abstract

The work is devoted to development of scanning imaging system for bioobjects based on two-photon fluorescence of nanoparticles. It is based on the use of nanomaterials in imagining systems and some of nonfluorescent imaging applications and labeling with fluorescent nanoparticles. The basics of two photon fluorescence, its imaging application, properties of nanoparticles and biological tissues, used for our system development were analyzed. Based on this analysis we developed the general scheme of the system for scanning imaging of bioobjects using CdTe quantum dot labels and method of two-photon fluorescence.