Image processing for semi-automated microscopic analysis of ice recrystallization process during isothermal annealing

Abstract

Investigation of the process of crystallization is a complex and important task, especially in cryopreservation of biological material for medical purposes. In the current work, the process of processing and analyzing a series of microscopic images during freezing and thawing is considered. Based on available literature as well as our image processing approach, it is now possible to analyze ice recrystallization process during thawing and isothermal annealing. Moreover, using our approach tracking of certain ice crystals can be performed. This knowledge can now be applied for precise determination of the efficiency of inhibition of ice recrystallization of novel and low-toxic cryoprotective agents.