Nanobiotechnology for health-care 
The investigation of dielectric constant of blood during sensitization to penicillin G
Soloshenko E.N.1, Kondakova A.K.1, Kolesnikov V.G.2, Khmil N.V.2, Shevchenko Z. M.1, Yarmak T.P.1 
1 SE «Institute of Dermatology and Venereology NAMS of Ukraine». Chernyshevskiy Str. 7/9, Kharkov-61057, Ukraine.
2  A. Ya. Usikov Institute for Radiophysics and Electronics  NAS  of Ukraine. Proskura  St., 12, Kharkov-61085, Ukraine. 
E-mail:dreval@ire.kharkov.ua  

The modern diagnostic algorithms for the detection of sensitization to penicillin G are aimed at the timely detection of an allergic reaction in order to prevent the development of serious life-threatening conditions of the human organism [1]. The microwave dielectrometry is advanced tool for nanodiagnostics of different physiological states on molecular and cellular levels because of possibilities determination of conformational changes of protein complexes in the field of free water dispersion. 
The aim of this work was to evaluate the effect of penicillin G on the patients’ blood with antibiotic sensitization in a history in vitro using the parameters of dielectric constant. To study the dielectric constant, venous blood was used in 23 patients with antibiotic sensitization between the ages of 35 and 50 years. Sensitization to penicillin G in these patients was confirmed by the method of leukocyte agglomeration reaction (LAR) and by estimating the erythrocyte sedimentation rate (ESR) loaded with the suspected allergen.
The citrate blood of the donors was mixed with saline in a 1:1 ratio and thoroughly mixed (control sample) to study the effect of penicillin G on the dielectric constant. In another test tube, citrated blood in a 1:1 ratio was mixed with an antibiotic solution at a concentration of 250 мg/ml (test sample) and then incubated at 37 ° C for 3 hours. It was found an increase in bound water in the test samples compared with the control. The obtained data closely correlate with the results of ESR - (r = 0.92) and LAR - (r = 0.89). The increase in hydration of macromolecular blood complexes in the presence of penicillin G is probably due to conformational changes in blood components under the action of the drug or an increase in the number of hydrate sites.
The high correlation coefficient of the obtained experimental data allows us to recommend the method of microwave dielectrometry for detecting of sensitization to drugs assessing the change in the integral state of blood hydration. 

1.	Canzani D., Aldeek F. Penicillin G’s function, metabolites, allergy, and resistance // J. Nutr. Hum. Health.- 2017.-1, N 1.-P. 28-40.